Middle East Respiratory Syndrome Coronavirus Nucleic Acid

Fa'amatalaga Puupuu:

O le pusa o loʻo faʻaaogaina mo le suʻesuʻeina lelei o le MERS coronavirus nucleic acid i totonu ole nasopharyngeal swabs ma Middle East Respiratory Syndrome (MERS) coronavirus.


Fa'amatalaga Oloa

Faailoga o oloa

Igoa o oloa

HWTS-RT031A-Middle East Respiratory Syndrome Coronavirus Nucleic Acid Detection Kit(Fluorescent PCR)

Epidemiology

Middle East respiratory syndrome coronavirus (MERS-CoV), o se β-coronavirus e mafua aifaʻamaʻi manava i tagata, na muaʻi iloa i le 60-tausaga le matua Saudi Arabian maʻi maʻi i le 24th Iulai, 2012. O le faʻataʻitaʻiga falemaʻi o le MERS-CoV faʻamaʻi pipisi mai le asymptomatic state poʻo le manava manava faʻamaʻi i le ogaoga o le manava manava e oʻo lava i le oti.

Auala

FAM MERS virus RNA
VIC(HEX)

Pulea i totonu

Vaega Fa'ainisinia

Teuina

≤-18℃ I le pogisa

Fa'asa-ola

9 masina

Ituaiga Fa'ata'ita'iga

Fufulu nasopharyngeal faatoa aoina

CV

≤5.0%

Ct

≤38

LoD

1000 Kopi/mL

Fa'apitoa

E leai se feso'ota'iga fa'atasi ma tagata coronaviruses tagata coronavirus SARSr-CoV ma isi fa'ama'i masani.

Mea faigaluega talafeagai:

Fa'aogaina Biosystems 7500 Fa'atonu PCR Taimi Moni

Fa'aogaina Biosystems 7500 Fa'atonuga PCR Taimi Moni Anapogi

QuantStudio®5 Faiga PCR Taimi Moni

SLAN-96P Faiga PCR Taimi Moni

LightCycler®480 Taimi moni PCR faiga

LineGene 9600 Plus Faiga Fa'amatalaga PCR Taimi Moni

MA-6000 Taimi Moni Fua Fa'ata'avale vevela

BioRad CFX96 Taimi Moni PCR System

BioRad CFX Opus 96 Taimi moni PCR System

Galulue Galuega

Filifiliga 1.

Fautuaina le fa'aputuina: QIAamp Viral RNA Mini Kit (52904), Nucleic Acid Extraction po'o le Purification Reagent (YDP315-R) e Tiangen Biotech (Beijing) Co., Ltd.

Filifiliga 2.

Fautuaina le fa'aaogaina o le fa'aputuga: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017) ma Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).


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