Human Papillomavirus (28 Ituaiga) Genotyping Detection Kit (Fluorescence PCR)

O lo'o fa'aogaina e le pusa le tele o auala e su'esu'eina ai le fela'ua'iga o le nucleic acid amplification (PCR).O fa'amatalaga ma'oti fa'apitoa ma su'esu'ega ua mamanuina e fa'atatau i le fa'asologa o kenera L1 o le HPV.O le suʻesuʻega faʻapitoa e faʻailogaina ile FAM fluorophore (HPV6, 16, 26, 40, 53, 58, 73), VIC / HEX fluorophore (HPV11, 18, 33, 43, 51, 59, 81), CY5 fluorophore (HPV35, 44). , 45, 54, 56, 68, 82) ma le ROX fluorophore (HPV31, 39, 42, 52, 61, 66, 83) i le 5', ma le vaega 3' quencher o le BHQ1 poʻo le BHQ2.I le taimi o le PCR fa'ateleina, o fa'amatalaga fa'apitoa ma su'esu'ega e fusifusia i latou fa'asologa fa'atatau.Pe a fetaiaʻi Taq enzyme i suʻesuʻega o loʻo noatia i le faʻasologa faʻasologa, e faʻaaogaina le galuega a le 5' end exonuclease e vavae ese ai le tusitala fluorophore mai le tineia fluorophore, ina ia mafai e le masini mataʻituina o le fluorescence ona maua le faailo o le fluorescent, o lona uiga, i taimi uma e DNA. strand ua faʻalauteleina, faʻatupuina se mole faʻafefete, lea e iloa ai le faʻamaopoopoina atoatoa o le faʻaputuina o faailoilo faʻamalama ma le faʻavaeina o oloa PCR, ina ia mafai ai ona maua le faʻamaonia ma le genotyping suʻesuʻega o nucleic acids o ituaiga 28 o papillomavirus tagata i faʻataʻitaʻiga cell exfoliated cervical. .