Pusa Su'esu'ega Genotyping Virus (Fluorescent PCR)
Igoa o oloa
HWTS-HP001-Hepatitis B Virus Genotyping Detection Kit (Fluorescent PCR)
Epidemiology
I le taimi nei, e sefulu ituaiga mai le A i le J o le HBV ua iloa i le lalolagi atoa.Eseese HBV genotypes e iai le eseesega i uiga faʻamaʻi, fesuiaiga o siama, faʻamaʻi faʻamaʻi ma tali togafitiga, ma isi, lea o le a aʻafia ai le HBeAg seroconversion rate, le ogaoga o manua o le ate, ma le aʻafiaga o le kanesa o le ate i se tulaga patino, ma aafia ai le falemaʻi. fa'ailoga o fa'ama'i pipisi o le HBV ma le aoga fa'afoma'i o vaila'au fa'ama'i i se tulaga patino.
Auala
| Auala Igoa | Tali Tali 1 | Tali Tali 2 |
| FAM | HBV-C | HBV-D |
| VIC/HEX | HBV-B | Pulea i totonu |
Tulaga Fa'ateleina PCR
| Laa | Ta'amilosaga | vevela | Taimi | Aoina Fa'ailoga Fluorescent pe Leai |
| 1 | 1 taamilosaga | 50℃ | 2 minu | No |
| 95℃ | 5 minu | No | ||
| 2 | 40 taamilosaga | 95℃ | 15 sec | No |
| 60℃ | 45 sekone | ioe |
Vaega Fa'atekinisi
| Teuga | ≤-18℃ I le pogisa |
| Fa'asa-ola | 12 masina |
| Ituaiga Fa'ata'ita'iga | Serum, Plasma |
| Ct | ≤38 |
| CV | ≤5.0％ |
| LoD | 1×102IU/mL |
| Meafaigaluega Faatatau | E mafai ona fetaui ma meafaifa'aili PCR fa'aulu i luga o le maketi.ABI 7500 Faiga PCR Taimi Moni ABI 7500 Fa'atonuga PCR Taimi Moni Anapogi SLAN-96P Faiga PCR Taimi Moni QuantStudio®5 Faiga PCR Taimi Moni LightCycler®480 Faiga PCR Taimi Moni LineGene 9600 Plus Faiga Fa'amatalaga PCR Taimi Moni MA-6000 Taimi Moni Fua Fa'ata'avale vevela BioRad CFX96 Taimi Moni PCR System BioRad CFX Opus 96 Taimi moni PCR System |
