Tagata Methylated NDRG4/SEPT9/SFRP2/BMP3/SDC2 Gene

Fa'amatalaga Puupuu:

O le pusa e faʻamoemoe mo in vitro suʻesuʻega faʻamaonia o le methylated NDRG4 / SEPT9 / SFRP2 / BMP3 / SDC2 genes i totonu o sela faʻamaʻi intestinal i faʻataʻitaʻiga o nofoa tagata.


Fa'amatalaga Oloa

Faailoga o oloa

Igoa o oloa

HWTS-OT077-Tagata Methylated NDRG4/SEPT9/SFRP2/BMP3/SDC2 Gene Su'esu'eina Kit (Fluorescence PCR)

Tusi Faamaonia

CE

Epidemiology

I tagata matutua, e sili atu nai lo le 10 8 intestinal epithelial sela e pa'u ese mai le puipui o le intestinal i aso uma, ma e faʻaumatia ma feces e ala i peristalsis tele bowel.Ona o sela tumo e sili atu ona pa'u ese mai le intestinal tract o le faʻalavelave faʻafuaseʻi, o le stool o tagata mamaʻi tumo intestinal o loʻo i ai le tele o sela faʻamaʻi ma mea e le masani ai, o le mea autu lea mo le suʻeina o nofoa mautu.O suʻesuʻega ua maua ai o le suiga o le methylation o gene promoters o se mea na tupu muamua i le tumorigenesis, ma o mea faʻapitoa e maua mai faʻataʻitaʻiga o le maʻi kanesa colorectal e mafai ona atagia ai le i ai o le kanesa i totonu o le manava i le taimi muamua.

NDRG4, faʻapitoa foi o le SMAP-8 ma le BDM1, o se tasi o sui e toafa o le NDRG gene family (NDRG1-4), lea na faʻaalia e fesoʻotaʻi ma le faʻateleina o le cell, faʻavasegaina, atinaʻe ma le faʻalavelave.O lo'o fa'amaonia o le NDRG4 methylation ose biomarker e mafai ona maua mo le su'esu'eina o le kanesa colorectal i fa'ata'ita'iga o le nofoa.

SEPT9 o se sui o le aiga o le Septin gene, lea e aofia ai le itiiti ifo ma le 13 genes e faʻapipiʻiina se vaega GTPase faʻasaoina e mafai ona fusifusia ai porotini e fesoʻotaʻi ma cytoskeleton, ma e fesoʻotaʻi ma le vaeluaga o sela ma le tumorigenesis.O suʻesuʻega ua maua ai o le methylated Septin9 gene content ua faʻatuputeleina le faʻateleina i faʻataʻitaʻiga o nofoa mai tagata mamaʻi e maua i le kanesa colorectal.

O polotini e feso'ota'i fa'alilo (sFRPs) fa'alilolilo o polotini solu lea o se vasega o fa'afeagai o le ala Wnt ona o le maualuga o le fa'aogaina o le fa'aogaina i le fa'afefete (Fz) mo le fa'ailoga Wnt.O le le fa'agaoioia o le gene SFRP e mafua ai le fa'agaoioia o le Wnt fa'ailoga e feso'ota'i ma le kanesa colorectal.I le taimi nei, o le SFRP2 methylation i totonu o le nofoa e mafai ona faʻaaogaina e avea o se biomarker e le faʻaleagaina mo le suʻesuʻeina o le kanesa colorectal.

O le BMP3 o se sui o le TGF-B superfamily ma o loʻo i ai se sao taua i le atinaʻeina o pepe e ala i le faʻaosoina ma le fausiaina o ponaivi vave.BMP3 e hypermethylated i le kanesa colorectal ma e mafai ona faʻaaogaina e fai ma faʻailoga taua ole tumo.

SDC2 o se cell surface heparan sulfate proteoglycan o loʻo aʻafia i le faʻatonutonuina o le tele o faʻagasologa o le physiological ma pathological.O le faʻaogaina o le tino e aofia ai le faʻateleina o le cell, faʻavasegaina, faʻapipiʻi, faʻalapotopotoga o le cytoskeletal, femalagaiga, faʻamalolo manuʻa, fesoʻotaʻiga cell-matrix, angiogenesis;Pathological faiga e aofia ai le mumu ma le kanesa.O le maualuga o le methylation o le SDC2 gene i aano o le kanesa colorectal na matua maualuga atu nai lo le mea masani.

Auala

Tali fa'afefe A

VIC/HEX methylated NDRG4 gene
ROX methylated SEPT9 gene
CY5 pulea totonu

Tali fa'afefe B

VIC/HEX methylated SFRP2 gene
ROX methylated BMP3 gene
FAM methylated SDC2 gene
CY5 pulea totonu

Fa'aliliuga

Kene

Alaala Fa'ailoga

Ct Taua

Fa'aliliuga

NDRG4

VIC (HEX)

Ct tau≤38

NDRG4 lelei

Ct tau>38 po'o lalo

NDRG4 leaga

SET9

ROX

Ct tau≤38

SEPT9 lelei

Ct tau>38 po'o lalo

SEPT9 leaga

SFRP2

VIC (HEX)

Ct tau≤38

SFRP2 lelei

Ct tau>38 po'o lalo

SFRP2 leaga

Vaega Fa'ainisinia

Teuina Suavai: ≤-18℃
Fa'asa-ola 9 masina
Ituaiga Fa'ata'ita'iga Fa'ata'ita'i Fa'ata'oto
CV ≤5.0%
Fa'apitoa E leai se fa'afeagai fa'afeagai ma le kanesa o le ate, kanesa o le ga'o, kanesa o lou tino ma le kanesa o le mama
Meafaigaluega Faatatau QuantStudio ®5 Faiga PCR Taimi Moni

SLAN-96P Faiga PCR Taimi Moni

LightCycler®480 Taimi moni PCR faiga

LineGene 9600 Plus Faiga Fa'amatalaga PCR Taimi Moni

MA-6000 Taimi Moni Fua Fa'ata'avale vevela

Galulue Galuega

Fautuaina le fa'aaogaina o le fa'aputuga: Macro & Micro-Test Viral DNA/RNA Kit(HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) ma Macro & Micro-Test Automatic Nucleic Acid Extractor(HWTS- 3006).


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