Coxsackie Virus Type A16 Nucleic Acid

Fa'amatalaga Puupuu:

O lenei pusa o loʻo faʻaogaina mo in vitro suʻesuʻega faʻamaonia o le Coxsackie virus type A16 nucleic acid i totonu o le faʻaʻi o tagata.


Fa'amatalaga Oloa

Faailoga o oloa

Igoa o oloa

HWTS-EV025-Coxsackie Virus Type A16 Nucleic Acid Detection Kit (Enzymatic Probe Isothermal Amplification)

Tusi Faamaonia

CE

Epidemiology

O lenei pusa o loʻo faʻaaogaina le Enzymatic Probe Isothermal Amplification (EPIA), ma mamanuina faʻamatalaga faʻapitoa ma RNA base-containing probes (rProbe) mo le itulagi sili ona faʻasaoina o Cox A16, ma faʻaopoopo le Bst enzyme ma le RNaseH enzyme i le taimi e tasi, lea o le agavale ma pito taumatau o le RNA faavae o le rProbe ua faailogaina i vaega fluorescent ma tineia, faasologa.Fa'aaoga le DNA polymerase activity ma le strand displacement activity of Bst enzyme e fa'alautele ai le sini e su'eina i se vevela faifaipea, e mafai e le enzyme RNaseH ona fa'amavaeina fa'avae RNA i luga o le filifili fa'atosina-su'esu'e, ina ia mafai ai e le kulupu fluorescent ma tineia o rProbe tuueseeseina faapea fluorescing.E le gata i lea, o le vaega agavale o le vaega totoe rProbe RNA e mafai ona faʻaaogaina e fai ma faʻamuamua e faʻalautele atili e fausia ai se oloa, lea e faʻaputu atili ai le oloa.O le faailo o le fluorescent o loʻo faʻaauau pea ona faʻaputuina i le faʻavaeina o le oloa, ma iloa ai le mauaina o le nucleic acid target.

Auala

FAM Coxsackie virus ituaiga A16
ROX fa'alavelave i totonu

Vaega Fa'ainisinia

Teuina

≤-18℃

Fa'asa-ola

12 masina

Ituaiga Fa'ata'ita'iga

Fufulu fa'a'i fou ua aoina

CV

≤10.0%

Ct

≤38

LoD

2000Kopi/mL

Meafaigaluega Faatatau

Fa'aogaina Biosystems 7500 Fa'atonu PCR Taimi MoniSLAN-96P Faiga PCR Taimi Moni

LightCycler®480 Taimi moni PCR faiga

Taimi moni Fluorescence Fa'asa'o Temperature Su'esu'ega System Faigofie Amp HWTS1600

Galulue Galuega

Filifiliga 1

Fautuaina le fa'aaogaina o le fa'aputuga: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) ma Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006)

Filifiliga 2

Fautuaina le fa'asusuina o mea fa'apipi'i: Macro & Micro-Test Sample Reagent Reagent (HWTS-3005-8).


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