Pusa RT-PCR fa'amalama fa'aola mo le su'eina o le SARS-CoV-2

Fa'amatalaga Puupuu:

O lenei pusa o loʻo faʻamoemoe e suʻesuʻe faʻamaonia le ORF1ab ma le N genes o le novel coronavirus (SARS-CoV-2) i totonu o le nasopharyngeal swab ma le oropharyngeal swab na aoina mai mataupu ma faʻapipiʻi mataupu masalomia i le novel coronavirus-infected pneumonia ma isi e manaʻomia mo le suʻesuʻeina. po'o su'esu'ega 'ese'ese o fa'ama'i fou o le coronavirus.


Fa'amatalaga Oloa

Faailoga o oloa

Igoa o oloa

HWTS-RT057A-Taimi mo'i felanulanua'i RT-PCR pusa mo le su'eina o le SARS-CoV-2

HWTS-RT057F-fa'a'aisa-mamago mo'i taimi fa'alelei RT-PCR kit mo le su'eina o le SARS-CoV-2 -Subpackage

Tusi Faamaonia

CE

Epidemiology

O le tala fou o le coronavirus (SARS-CoV-2) ua salalau atu i se tulaga tele i le lalolagi atoa.I le faagasologa o le faʻasalalauga, o suiga fou e tupu i taimi uma, e mafua ai suiga fou.O lenei oloa e masani ona faʻaaogaina mo le suʻesuʻeina fesoasoani ma le faʻavasegaina o mataupu e fesoʻotaʻi ma faʻamaʻi pe a maeʻa le faʻasalalau tele o le Alpha, Beta, Gamma, Delta ma Omicron mutant strains talu mai Tesema 2020.

Auala

FAM 2019-nCoV ORF1ab gene
CY5 2019-nCoV N gene
VIC(HEX) gene faasinomaga totonu

Vaega Fa'ainisinia

Teuina

Suavai: ≤-18℃ I le pogisa

Lyophilized: ≤30 ℃ I le pogisa

Fa'asa-ola

Suavai: 9 masina

Lyophilized: 12 masina

Ituaiga Fa'ata'ita'iga

fa'asu'u nasopharyngeal, su'ega oropharyngeal

CV

≤5.0%

Ct

≤38

LoD

300Kopi/mL

Fa'apitoa

E leai se felafolafoaʻiga faʻatasi ma tagata coronavirus SARS-CoV ma isi faʻamaʻi masani.

Mea faigaluega talafeagai:

Fa'aogaina Biosystems 7500 Fa'atonu PCR Taimi Moni

Fa'aogaina Biosystems 7500 Fa'atonuga PCR Taimi Moni Anapogi

SLAN ®-96P Faiga PCR Taimi Moni

QuantStudio™ 5 Faiga PCR Taimi Moni

LightCycler®480 Taimi moni PCR faiga

LineGene 9600 Plus Faiga Fa'amatalaga PCR Taimi Moni

MA-6000 Taimi Moni Fua Fa'ata'avale vevela

BioRad CFX96 Taimi Moni PCR System

BioRad CFX Opus 96 Taimi moni PCR System

Galulue Galuega

Filifiliga 1.

Nucleic Acid Extraction poʻo le Pusa Faʻamamā (Metotia Pepa maneta)(HWTS-3001, HWTS-3004-32, HWTS-3004-48) mai le Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.

Filifiliga 2.

Fautuaina le fa'aaogaina o mea fa'apipi'i: QIAamp Viral RNA Mini Kit (52904), Viral RNA Extraction Kit (YDP315-R) gaosia e Tiangen Biotech (Beijing) Co., Ltd.


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