Mycobacterium Tuberculosis Rifampicin Resistance

Fa'amatalaga Puupuu:

O lenei pusa e fetaui lelei mo le suʻesuʻeina lelei o le suiga o le homozygous i le 507-533 amino acid codon region o le rpoB gene e mafua ai le Mycobacterium tuberculosis rifampicin resistance.


Fa'amatalaga Oloa

Faailoga o oloa

Igoa o oloa

HWTS-RT074A-Mycobacterium Tuberculosis Rifampicin Resistance Detection Pusa (Fluorescence PCR)

Epidemiology

O le Rifampicin na fa'aaogaina lautele i togafitiga o gasegase mamapala talu mai le tuai o 1970s, ma e iai sona aafiaga taua.O le filifiliga muamua lava e fa'apu'upu'u le chemotherapy o tagata mama'i mamapala.O le tetee i le Rifampicin e mafua ona o le suiga o le kenera rpoB.E ui lava o loʻo faʻaauau pea ona faʻaalia fualaau fou e tetee atu ai i le mamapala, ma o loʻo faʻaauau pea le faʻaleleia atili o le aoga o tagata mamaʻi mamaʻi, o loʻo i ai pea le le lava o vailaʻau faʻamaʻi, ma o le tulaga o le faʻaogaina o vailaʻau le talafeagai i falemaʻi e fai si maualuga.E manino lava, o le Mycobacterium tuberculosis i tagata mamaʻi e maua i le mama mama e le mafai ona faʻaumatia atoa i se taimi talafeagai, lea e iu ai ina oʻo atu i tikeri eseese o vailaʻau faʻasaina i le tino o le tagata maʻi, faʻaumiina le ala o le faʻamaʻi, ma faʻateleina ai le lamatiaga o le oti o le tagata maʻi.O lenei pusa e fetaui lelei mo suʻesuʻega fesoasoani o le Mycobacterium tuberculosis faʻamaʻi ma le suʻesuʻeina o le rifampicin resistance gene, lea e fesoasoani e malamalama ai i le teteʻe o vailaʻau o le mycobacterium tuberculosis e aʻafia e tagata mamaʻi, ma tuʻuina atu auala fesoasoani mo le taʻitaʻiga o vailaʻau.

Vaega Fa'ainisinia

Teuina

≤-18℃ I le pogisa

Fa'asa-ola

9 masina

Ituaiga Fa'ata'ita'iga

Sputum

CV

≤5.0%

LoD

ituaiga vao e tetee i le rifampicin: 2x103siama/mL

mutant homozygous: 2x103siama/mL

Fa'apitoa

O lenei pusa e leai se fa'afeagai fa'atasi ma genome tagata, isi mycobacteria e le tuberculous, ma fa'ama'i niumonia.E iloa ai le suiga o nofoaga o isi vailaʻau teteʻe genes o vao-ituaiga mycobacterium tuberculosis e pei o katG 315G>C\A, InhA-15C> T, o le suʻega iʻuga o loʻo faʻaalia ai le leai o se tetee i le rifampicin, o lona uiga e leai se tali faʻasaga.

Mea faigaluega talafeagai:

SLAN-96P Faiga PCR Taimi Moni

Galulue Galuega

Filifiliga 1.

Fautuaina le fa'aaogaina o le fa'aputuga: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) ma Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).

Filifiliga 2.

Fautuaina le fa'aputuina o mea fa'aputu: Nucleic Acid Extraction po'o le Purification Reagent(YDP302) e Tiangen Biotech(Beijing) Co.,Ltd.


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