Mycobacterium Tuberculosis Isoniazid Resistance Mutation

Fa'amatalaga Puupuu:

O lenei pusa e fetaui lelei mo suʻesuʻega faʻapitoa o nofoaga autu o suiga i faʻataʻitaʻiga sputum tagata na aoina mai Tubercle bacillus maʻi lelei e taʻitaʻia ai le mycobacterium tuberculosis isoniazid resistance: InhA promoter region -15C>T, -8T>A, -8T>C;AhpC fa'alauiloa itulagi -12C>T, -6G>A;suiga homozygous o KatG 315 codon 315G>A, 315G>C .

Auina imeli ia matou

Fa'amatalaga Oloa

Faailoga o oloa

Igoa o oloa

HWTS-RT137 Mycobacterium Tuberculosis Isoniazid Resistance Mutation Detection Kit (Meli Liusua)

Epidemiology

O le Mycobacterium tuberculosis, e pei o le Tubercle bacillus (TB), o le siama faʻamaʻi e mafua ai le mamapala.I le taimi nei, o vailaʻau faʻamaʻi faʻamaʻi e masani ona faʻaaogaina muamua e aofia ai isoniazid, rifampicin ma hexambutol, ma isi. Ae ui i lea, ona o le le saʻo o le faʻaogaina o vailaʻau faʻamaʻi faʻamaʻi ma uiga o le fausaga puipui puipui o le mycobacterium tuberculosis, o le mycobacterium tuberculosis e atiaʻe ai le teteʻe o vailaʻau i vailaʻau faʻamaʻi, lea e aumaia ai luitau ogaoga i le puipuia ma le togafitia o le mamapala.

Auala

FAM	MP nucleic acid
ROX	Pulea i totonu

Vaega Fa'ainisinia

Teuina	≤-18℃
Fa'asa-ola	12 masina
Ituaiga Fa'ata'ita'iga	fuga
CV	≤5.0%
LoD	Ole tapula'a ole su'esu'ega ole siama ole isoniazid ile vao ole 2x103 siama/mL, ma le tapula'a e iloa ai siama mutant ole 2x103 siama/mL.
Fa'apitoa	a.E leai se fa'afeagai fa'afeagai i le genome o le tagata, isi fa'ama'i mycobacteria e le tuberculous ma le niumonia o lo'o maua e lenei pusa. e.O nofoaga o suiga o isi genes e tetee atu i fualaau oona i le Mycobacterium tuberculosis, e pei o le vaega e filifili ai le tetee o le kenera rifampicin rpoB, na maua, ma o taunuuga o suʻega na faʻaalia ai le leai o se tetee i le isoniazid, e faʻaalia ai le leai o se gaioiga faʻafefe.
Meafaigaluega Faatatau	SLAN-96P Faiga PCR Taimi Moni BioRad CFX96 Faiga PCR Taimi Moni LightCycler480® Faiga PCR Taimi Moni

Galulue Galuega

Afai e faʻaaoga le Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (lea e mafai ona faʻaaogaina i le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) e Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. mo le mauaina, faaopoopo le 200μL ole fa'atonuga leaga ma le fa'ata'ita'iga ole sputum e fa'ata'ita'iina ile fa'asologa, ma fa'aopoopo le 10μL o le pulea totonu eseese i le pulea lelei, faʻataʻitaʻiga sputum faʻataʻitaʻiina e faʻataʻitaʻiina, ma o laasaga mulimuli ane e tatau ona faʻataunuʻuina e tusa ai ma faʻatonuga.O le voluma fa'ata'ita'i ua maua e 200μL, ma o le voluma elution fautuaina e 100μL.