MTHFR Gene Polymorphic Nucleic Acid
Igoa o oloa
HWTS-GE004-MTHFR Gene Polymorphic Nucleic Acid Detection Kit (ARMS-PCR)
Epidemiology
O le folic acid o se vaitamini e solu vai ma ose fesoasoani taua i ala fa'atosina o le tino.I tausaga talu ai nei, o le tele o suʻesuʻega ua faʻamaonia ai, o le suiga o le folate metabolizing enzyme gene MTHFR o le a taʻitaʻia ai le le lava o le folic acid i le tino, ma o le faʻaleagaina masani o le folic acid deficiency i tagata matutua e ono mafua ai le anemia megaloblastic, vascular. endothelial faaleagaina, ma isi. Folic acid deficiency i fafine maʻitaga e le mafai ona ausia manaoga o latou lava ma le fetus, e mafai ona mafua ai le faaletonu o le paipa neural, anencephaly, fanau pepe, ma le faʻaipoipo.Ole maualuga ole folate ole toto e a'afia ile 5,10-methylenetetrahydrofolate reductase (MTHFR) polymorphisms.O suiga ole 677C>T ma le 1298A>C i le MTHFR gene e mafua ai le liua o le alanin i le valine ma le glutamic acid, i le faasologa, e mafua ai le faʻaitiitia o le MTHFR gaioiga ma faʻaitiitia ai le faʻaogaina o le folic acid.
Auala
FAM | MTHFR C677T |
ROX | MTHFR A1298C |
VIC(HEX) | Pulea totonu |
Vaega Fa'ainisinia
Teuina | ≤-18℃ |
Fa'asa-ola | 12 masina |
Ituaiga Fa'ata'ita'iga | Toto fa'ato'a fa'ato'a fa'ato'a fa'ato'a aoina mai EDTA |
CV | ≤5.0% |
Ct | ≤38 |
LoD | 1.0ng/μL |
Mea faigaluega talafeagai: | Fa'aogaina Biosystems 7500 Fa'atonu PCR Taimi Moni Fa'aogaina Biosystems 7500 Fa'atonuga PCR Taimi Moni Anapogi SLAN ®-96P Faiga PCR Taimi Moni QuantStudio™ 5 Faiga PCR Taimi Moni LightCycler®480 Taimi moni PCR faiga LineGene 9600 Plus Faiga Fa'amatalaga PCR Taimi Moni MA-6000 Taimi Moni Fua Fa'ata'avale vevela BioRad CFX96 Taimi Moni PCR System BioRad CFX Opus 96 Taimi moni PCR System |
Galulue Galuega
Filifiliga 1
Reagents fa'atonuina: Macro & Micro-Test Genomic DNA Kit (HWTS-3014-32, HWTS-3014-48, HWTS-3014-96) ma Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B) .
Filifiliga 2
Fautuaina le fa'aputuina o mea: Blood Genomic DNA Extraction Kit(YDP348, JCXB20210062) e Tiangen Biotech(Beijing) Co.,Ltd.Toto Genome Extraction Kit(A1120) saunia e Promega.