Tagata BRAF Gene V600E Suiga
Igoa o oloa
HWTS-TM007-Tagata BRAF Gene V600E Su'esu'ega Pusa (Fluorescence PCR)
Tusi Faamaonia
CE
Epidemiology
E sili atu i le 30 ituaiga o suiga o le BRAF ua maua, lea e tusa ma le 90% o loʻo iai i le exon 15, lea o le V600E mutation ua manatu o le suiga sili ona taatele, o lona uiga, thymine (T) i le tulaga 1799 i le exon 15 ua suia i le adenine (A), e mafua ai le suia o le valine (V) i le tulaga 600 e le glutamic acid (E) i le oloa porotini.O suiga ole BRAF e masani ona maua ile tuma ma'i e pei ole melanoma, kanesa colorectal, kanesa lauroid, ma kanesa mama.O le malamalama i le suiga ole gene BRAF ua avea ma mana'oga e su'e le EGFR-TKIs ma le BRAF gene mutation-targeted drugs in clinical targeted drug therapy mo tagata mama'i e mafai ona manuia.
Auala
| FAM | V600E suiga, pulea totonu |
Vaega Fa'ainisinia
| Teuina | ≤-18℃ |
| Fa'asa-ola | 9 masina |
| Ituaiga Fa'ata'ita'iga | fa'ata'ita'iga o a'afiaga fa'ama'i fa'apipi'i paraffin |
| CV | <5.0% |
| Ct | ≤38 |
| LoD | Fa'aoga pusa e iloa ai le fa'atonuga lelei o le LoD.a) i lalo ole 3ng/μL vao-ituaiga talaaga, 1% fua faatatau mutation e mafai ona iloa i le tali pa stably;b) i lalo o le 1% fua faatatau suiga, le suiga o le 1 × 103Kopi/mL ile fa'ata'ita'iga o le vao-ituaiga 1×105Kopi/mL e mafai ona iloa lelei i le tali fa'apolopolo;c) e mafai e le IC Reaction Buffer ona iloa le fa'atapula'aina o le fa'atapula'aina o le fa'atonuga lelei SW3 a le kamupani i totonu. |
| Mea faigaluega talafeagai: | Fa'aogaina Biosystems 7500 Fa'atonu PCR Taimi MoniApplied Biosystems 7300 Real-Time PCR Faiga, QuantStudio® 5 Fa'atonu PCR Taimi Moni LightCycler®480 Taimi moni PCR faiga BioRad CFX96 Taimi Moni PCR System |
Galulue Galuega
Fa'atonuga fa'afo'esega: QIAGEN's QIAamp DNA FFPE Tissue Kit (56404), Paraffin-embedded Tissue DNA Rapid Extraction Kit (DP330) gaosia e Tiangen Biotech(Beijing) Co., Ltd.
