Herpes Simplex Virus Type 1/2,(HSV1/2) Nucleic Acid
Igoa o oloa
HWTS-UR018A-Herpes simplex virus type 1/2, (HSV1/2) pusa su'esu'e o le nucleic acid (Fluorescence PCR)
Epidemiology
O faʻamaʻi pipisi mai feusuaiga (STD) o loʻo avea pea ma se tasi o faʻamataʻu tele i le saogalemu o le soifua maloloina lautele o le lalolagi.O ia faʻamaʻi e mafai ona oʻo atu ai i le le maua o fanau, faʻataʻitaʻiina o le fetal, tumo ma faʻafitauli matuia eseese.E tele ituaiga o STD pathogens, e aofia ai siama, viruses, chlamydia, mycoplasma ma spirochetes, e aofia ai Neisseria gonorrhoeae, Mycoplasma genitalium, Chlamydia trachomatis, HSV1, HSV2, Mycoplasma hominis, ma Ureaplasma urealyticum e taatele.
O le fa'ama'i fa'afeusuaiga ose fa'ama'i pipisi fa'afeusuaiga e mafua mai ile HSV2, lea e sili ona fa'ama'i.I tausaga talu ai nei, o le aʻafiaga o le herpes genital ua matua faʻateleina, ma ona o le faʻateleina o amioga faʻafeusuaiga mataʻutia, o le maualuga o le iloa o le HSV1 i le herpes genital ua faʻateleina ma lipotia mai e maualuga i le 20% -30%.O se fa'ama'i muamua i le fa'ama'i o le genital herpes e tele lava ina le leoa e aunoa ma ni fa'ailoga fa'apitoa fa'apitoa se'i vagana herpes i le lotoifale i le mucosa po'o le pa'u o nai gasegase.Talu ai ona o le herpes genital e faʻaalia i le faʻamaligiina o viral i le olaga atoa ma le faʻaalia i le toe tupu, e taua le siakiina o siama i se taimi vave e mafai ai ma poloka lona feaveaʻi.
Auala
FAM | HSV1 |
CY5 | HSV2 |
VIC(HEX) | Pulea i totonu |
Vaega Fa'ainisinia
Teuina | Suavai: ≤-18℃ I le pogisa |
Fa'asa-ola | 12 masina |
Ituaiga Fa'ata'ita'iga | urethral secretions, fa'alilo fa'atagata |
Ct | ≤38 |
CV | ≤5.0% |
LoD | 50 Kopi/tali |
Fa'apitoa | E leai se feso'ota'iga fa'atasi ma isi fa'ama'i STD e pei o Treponema pallidum, Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma hominis, Mycoplasma genitalium, ma Ureaplasma urealyticum. |
Meafaigaluega Faatatau | E mafai ona fetaui ma meafaifa'aili PCR fa'aulu i luga o le maketi. Fa'aogaina Biosystems 7500 Fa'atonuga PCR Taimi Moni Anapogi QuantStudio®5 Faiga PCR Taimi Moni SLAN-96P Faiga PCR Taimi Moni LightCycler®480 Taimi moni PCR faiga LineGene 9600 Plus Faiga Fa'amatalaga PCR Taimi Moni MA-6000 Taimi Moni Fua Fa'ata'avale vevela BioRad CFX96 Taimi Moni PCR System BioRad CFX Opus 96 Taimi moni PCR System |
Galulue Galuega
Filifiliga 1.
Fautuaina le fa'aaogaina o le fa'aputuga: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) ma Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).
Filifiliga 2.
Fautuaina le fa'aputuina o mea fa'aputu: Nucleic Acid Extraction po'o le Purification Reagent(YDP302) e Tiangen Biotech(Beijing) Co.,Ltd.