Chlamydia Trachomatis Nucleic Acid
Igoa Oloa
HWTS-UR001A-Chlamydia Trachomatis Pusa Su'esu'ega Nucleic Acid (Fluorescence PCR)
Fa'aoga Fa'amoemoe
O lenei pusa o loʻo faʻaogaina mo le suʻesuʻeina lelei o le Chlamydia trachomatis nucleic acid i le mimi tane, tamaʻitaʻi urethral swab, ma tamaʻitaʻi faʻataʻitaʻiga swab.
Epidemiology
Chlamydia trachomatis (CT) o se ituaiga o microorganism prokaryotic e matua faʻamaʻi i sela eukaryotic.Chlamydia trachomatis ua vaevaeina i AK serotypes e tusa ai ma le auala serotype.O faʻamaʻi pipisi o le urogenital e tele lava ina mafua mai i le trachoma biological variant DK serotypes, ma o tane e tele lava ina faʻaalia e pei o le urethritis, lea e mafai ona faʻamalolo e aunoa ma se togafitiga, ae o le tele oi latou e avea ma taimi masani, faʻalavelave faʻafuaseʻi, ma e mafai ona tuʻufaʻatasia ma epididymitis, proctitis, ma isi. e mafai ona mafua mai i le urethritis, cervicitis, ma isi, ma faʻalavelave sili atu ona ogaoga o le salpingitis.
Epidemiology
FAM: Chlamydia trachomatis (CT)·
VIC(HEX): Pulea totonu
PCR Fa'aopoopo Tulaga Fa'atonu
Laa | Ta'amilosaga | vevela | Taimi | Aoina Fa'ailoga Fluorescent pe Leai |
1 | 1 taamilosaga | 50℃ | 5min | No |
2 | 1 taamilosaga | 95℃ | 10min | No |
3 | 40 taamilosaga | 95℃ | 15 sec | No |
4 | 58℃ | 31 sekone | Ioe |
Vaega Fa'ainisinia
Teuina | |
Suavai | ≤-18℃ I le pogisa |
Fa'asa-ola | 12 masina |
Ituaiga Fa'ata'ita'iga | Fa'alilo fa'atama'i, Fa'amama fa'atama'ita'i, Mimi tane |
Ct | ≤38 |
CV | ≤5.0% |
LoD | 50Kopi/tali |
Fa'apitoa | E leai se fa'alavelave fa'asagatau mo le su'eina o isi fa'ama'i fa'ama'i STD e lenei pusa, e pei o Treponema pallidum, Neisseria gonorrhoeae, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasma genitalium, ma isi, o lo'o i fafo atu o le va'aiga o pusa. |
Meafaigaluega Faatatau | E mafai ona fetaui ma meafaifa'aili PCR fa'aulu i luga o le maketi. Fa'aogaina Biosystems 7500 Fa'atonu PCR Taimi Moni Fa'aogaina Biosystems 7500 Fa'atonuga PCR Taimi Moni Anapogi QuantStudio® 5 Faiga PCR Taimi Moni SLAN-96P Faiga PCR Taimi Moni LightCycler®480 Taimi moni PCR faiga LineGene 9600 Plus Faiga Fa'amatalaga PCR Taimi Moni MA-6000 Taimi Moni Fua Fa'ata'avale vevela BioRad CFX96 Taimi Moni PCR System BioRad CFX Opus 96 Taimi moni PCR System |