28 Ituaiga o le Human Papilloma Virus (16/18 Tu'aiga) Nucleic Acid
Igoa o oloa
HWTS-CC006A-28 Ituaiga o Fa'ama'i Papilloma Fa'atagata Mata'utia (16/18 Ta'itusi) Pusa Su'esu'ega Nucleic Acid (Fluorescence PCR)
Epidemiology
O le kanesa fa'a'au'au o se tasi lea o tuma ma'i e sili ona taatele o le fa'atamatama fafine.O su'esu'ega ua fa'aalia ai o fa'ama'i pipisi o le HPV ma le tele o fa'ama'i ose tasi lea o mafua'aga autu o le kanesa fa'a'a'au.I le taimi nei, o lo'o fa'ailoa mai e le'o maua ni togafitiga lelei mo le kanesa fa'a'a'au e mafua mai i le HPV, o lea o le vave maua ma le puipuia o fa'ama'i fa'ama'i e mafua mai i le HPV o le ki lea e puipuia ai le kanesa fa'a'a'au.E taua tele le fa'atuina o se su'esu'ega fa'apitoa faigofie, ma'oti ma vave mo le su'esu'eina o le fa'ama'i ma togafitiga o le kanesa fa'a'a'au.
Auala
| Faafefiloiga Tali | Auala | Ituaiga |
| PCR-Mix1 | FAM | 18 |
| VIC(HEX) | 16 | |
| ROX | 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 | |
| CY5 | Pulea i totonu | |
| PCR-Mix2 | FAM | 6, 11, 54, 83 |
| VIC(HEX) | 26, 44, 61, 81 | |
| ROX | 40, 42, 43, 53, 73, 82 | |
| CY5 | Pulea i totonu |
Vaega Fa'ainisinia
| Teuina | Suavai: ≤-18℃ |
| Fa'asa-ola | 12 masina |
| Ituaiga Fa'ata'ita'iga | sela fa'a'a'au |
| Ct | ≤28 |
| CV | ≤5.0% |
| LoD | 300Kopi/mL |
| Meafaigaluega Faatatau | Fa'aogaina Biosystems 7500 Fa'atonu PCR Taimi Moni QuantStudio®5 Faiga PCR Taimi Moni SLAN-96P Faiga PCR Taimi Moni LightCycler®480 Faiga PCR Taimi Moni LineGene 9600 Plus Faiga Fa'amatalaga PCR Taimi Moni MA-6000 Taimi Moni Fua Fa'ata'avale vevela BioRad CFX96 Taimi Moni PCR System BioRad CFX Opus 96 Taimi moni PCR System |
Galulue Galuega
Filifiliga 1.
Reagents fa'atonuina: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48), ma Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).
Filifiliga 2.
Fa'atonuga fa'ameamea: Nucleic Acid Extraction po'o le Purification Kit(YDP315) e Tiangen Biotech (Beijing) Co.,Ltd.
